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Millipore/FCCS025101 | FlowCellect? EGFR/MAPK Pathway Activation Detection Kit/FCCS025101/25 Tests / Kit
  • Millipore/FCCS025101 | FlowCellect? EGFR/MAPK Pathway Activation Detection Kit/FCCS025101/25 Tests / Kit

Millipore/FCCS025101 | FlowCellect? EGFR/MAPK Pathway Activation Detection Kit/FCCS025101/25 Tests / Kit

價(jià)格: ￥6240.00 市場(chǎng)價(jià): 10400.00
貨號(hào): FCCS025101
品牌: Millipore
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    • Description
      CatalogueNumberFCCS025101
      TradeName
      • FlowCellect
      DescriptionFlowCellect?EGFR/MAPKPathwayActivationDetectionKit
      OverviewMillipore’sFlowCellect?EGFR/MAPKActivationDetectionkitincludestwodirectlyconjugatedphosphospecificantibodies:pEGFR(Tyr1173)-AlexaFluor488andpERK(Thr202/Tyr204,Thr185/Tyr187)-PE,alongwithoptimizedfixation,permeABIlization,washandassaybufferstoprovideresearcherstheabilitytodetecttheactivationofEGFRandERKatthereceptoranddownstreamsignalinglevel.
      TheabilitytodetectthephosphorylationofEGFRandERKsimultaneouslyallowsresearcherstoprofileEGFmediatedMAPKactivationwithinacelltypeofinterest.Alterationsinthesesignalingprofilescanbeusedtomonitortheeffectofaparticularupstreamtreatmenttargetingthepathway.
      Antibodiesagainstphospho-EGFRandphospho-ERK1/2areprovidedinthekittomeasuretheextentofeachproteinsactivationstate.A431cellswereusedasamodelsystemtodemonstrateEGFmediatedactivationofEGFRaswellasits’downstreameffectorkinaseERK.Thetreatedcellswerecomparedtountreatedcellstoverifythedetectionofbothactivatedproteinsbyflowcytometry.Whenperformingadualcolorstain,themajorityofthetreatedcellsaredoublepositiveindicatingactivationofMAPKthroughEGFR.
      Sufficientreagentsareprovidedfor253-colorsamples.Thekitincludesalloptimizedfluorescentlylabeledantibodiesandbuffersnecessaryforcellpreparationandanalysis.
      AlternateNames
      • FlowCellect
      BackgroundInformationTheEpidermalGrowthFactorReceptor(EGFR)isoneoftheReceptorTyrosineKinaseswhichcanactivatetheMAPKpathway,oneofthreemajorsignalingpathwaysdownstreamofEGFR.EGFRbelongstoErbBreceptorsuperfamily,agroupoftransmembraneglycoproteinreceptors,consistingoffourmembers:EGFR(ErbB-1),ErbB-2(c-neu,HER2),ErbB-3(HER3),andErbB-4(HER4).

      Criticalcellulardecisionssuchasproliferation,migration,anddifferentiation,areregulatedby
      stimulatorycuesfromtheextracellularenvironment,primarilygrowthfactors,extracellular
      matrix(ECM)proteins,andadhesionmoleculespresentedonthesurfaceofneighboringcells.Oncethesecuesarereceived,cellsundergocomplexsignalingcascadessuchastheMitogenActivatedProteinKinase(MAPK)pathwayinordertorespondtotheirenvironmentalstimuli.

      GrowthfactorsandmitogensusetheRas/Raf/MEK/ERKsignalingcascadetotransmitsignalsfrom
      theirreceptorstoregulategeneexpressionandpreventapoptosis.OnceactivatedbyphosphorylationExtra-cellularsignalRelatedKinase(ERK)entersthenucleusandactivatestargetproteinsassociatedwithcellularproliferationandmigration.

      DeregulationoftheEGFR/ERKpathwayduetoalterationsaffectingtheexpressionorfunctionofanumberofpathwaycomponentshaslongbeenassociatedwithnumerousformsofcancer.DuetothecomplexityofthepathwayanditsinvolvementinmanymalignanciestheEGFR/MAPKpathwayisstudiedbynumerousresearcherslookingforinsightintothemechanismsinvolvedintheformationandprogressionofcancers.
      ProductInformation
      Components
      • 1.20XAnti-phospho-EGFR(Tyr1173),clone9H2;AlexaFluor488Conjugate:(PartNo.CS204825)Onevialcontaining150mLantibody.
      • 2.20XAnti-phospho-ERK1/2(Thr202/Tyr204,Thr185/Tyr187),cloneAW39R;R-PhycoerythrinConjugate:(PartNo.CS203329)Onevialcontaining150mLantibody.
      • 3.FixationBuffer:(PartNo.CS202122)Onebottlecontaining13mLbuffer.
      • 4.10XWashBuffer:(PartNo.CS202123)Onebottlecontaining13mLbuffer.
      • 5.5XAssayBuffer:(PartNo.CS202124)Onebottlecontaining55mLbuffer.
      • 6.1XPermeabilizationBuffer:(PartNo.CS202125)Onebottlecontaining13mLbuffer.
      DetectionmethodFluorescent
      StorageandShippingInformation
      StorageConditions4-8°Cforantibodiesandbuffers
      Applications
      ApplicationThisFlowCellectEGFR/MAPKActivationDetectionKitforFlowCytometryprovidesresearcherstheabilitytodetecttheactivationofEGFR&ERKatthereceptor&downstreamsignalinglevel.
      KeyApplications
      • FlowCytometry
      BIOLOGicalInformation
      Clone9H2;AW39R
      HostMouse
      SpeciesReactivity
      • Human
      AntibodyTypeMonoclonalAntibody
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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