| CatalogueNumber | ECM200 | 
| TradeName |  | 
| Description | QCM3μmEndothelialCellMigrationAssayFibronectin,Colorimetric | 
| Overview | Alsoavailable:CellComb?ScratchAssay!Getbiochemicaldatafromascratchassay!ClickHere 
 Introduction
 Angiogenesisisafundamentalprocessinvolvingthegrowthofnewbloodvesselsfrompre-existingvessels.Itisimportantindevelopmentandwoundhealing,aswellaspathologicdiseasessuchasdiabeticretinopathyandcancer.Duringangiogenesis,endothelialcellsneedtomoveoutofexistingvessels,migrateintonewareas,proliferateandassembleintonewcapillaries.Themigrationofendothelialcellsisregulatedbymanyangiogenicfactorsandanti-angiogenicfactors.Itiscriticalforresearcherstounderstandthemechanismsofendothelialcellmigration.
 
 Millipore"s3μmQCM?EndothelialCellMigrationAssay–Fibronectin,ColorimetricprovidesaquickandefficientsystemtostudytheABIlityofcompoundstoinduceorinhibitendothelialcellmigration.Thisassayalsoallowsscreeningofpharmacologicalagents,evaluationofintegrinsorotheradhesionreceptorsresponsIBLeforendothelialcellmigration,analysisofgenefunctionintransfectedcells,anddeterminationofECMproteininvolvementincellmovement.
 
 Thisversatileassaypermitscountingofindividualmigratorycells,and,moreimportantly,allowsquantitativeanalysisbyopticaldensity(OD)usingastandardmicroplatereader.Thisconvenientassayallowslargescalescreeningandquantitativecomparisonofmultiplesamplesandincludesindividualmigrationcontrolsforeachsample.
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| MaterialsRequiredbutNotDelivered | 1.PrecisionPipettes:sufficientforaliquotingcells. 2.Harvestingbuffer:EDTAortrypsinbasedcelldetachmentbuffer,orothercelldetachmentformulationsasoptimizedbyindividualinvestigators.Millipore’sready-to-usenon-mammaliandetachmentsolution,Accutase?(Cat.No.SCR005)canalsobeused.
 3.Endothelialcells(forexample:HUVECcells).
 4.Endotheliumcellculturemediumappropriateforsubjectcells,suchasEGM-2(Endothelialcellgrowthmedia-2)
 5.QuenchingMedium:serum-freemedium,suchasDMEM,MEMetccontaining5%BSA.Mustcontaindivalentcations(Mg2+,Ca2+)sufficientforquenchingEDTAinharvestingbuffer.
 6.SterilePBSorHBSStowashcells.
 7.Distilledwater
 8.(Optional)Chemoattractantorpharmacologicalagentaddedtoculturemedium.
 9.Lowspeedcentrifugeandtubesforcellharvesting.
 10.CO2incubatorappropriateforsubjectcells.
 11.Hemocytometerorothermeansofcountingcells.
 12.Trypanblueorequivalentviabilitystain.
 13.Microplatereader(540-570nmdetection)orspectrophotometer.
 14.Sterilecellculturehood
 15.(Optional)Graduatedocular(calibrated),orautomatedmethodforcountingstainedcellsonamembrane.
 16.Shaker
 
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