The antiserum was produced against synthesized peptide derived from human AMPK1 around the phosphorylation site of Ser496.
Immunogen Range
451-500
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
AMPK1 (Phospho-Ser496) Antibody detects endogenous levels of AMPK1 only when phosphorylated at Ser496.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
AAPK1
Database Links
Gene ID: 5562 Swiss-Prot #: Q13131
OMIM #: 602739 Unigene #: Hs.43322
Target Modification
Phospho
Modification Sites
H:S496 M:S496 R:S496
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using AMPK1 (Phospho-Ser485) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HeLa cells treated with heat shock, using AMPK1 (Phospho-Ser485) Antibody. The lane on the right is blocked with the phospho peptide.